Between Catalase from a Potato and Hydrogen Peroxide The dependent variable the one I intend to measure is the volume of gas produced in each reaction. As the substrate and the inhibitor are not competry for the same site, an increase in substrate concentration does not decrease the effect of inhibitors. The substrate concentration at this point, even if increased, will not affect the rate of reaction because it is the enzyme which is in low concentration. Obtained 20 test tubes and 4 test tube rack 2. Extension Work This experiment could be improved in a number of ways.

So there are more collisions between substrate hydrogen peroxide and enzyme catalase active site, and more products will be formed water and oxygen. This type of enzyme accelerates reactions in which a substrate is broken down into simpler compounds through reaction with adding up water molecules. They are all very specific as each enzyme just performs one particular reaction. I will work out the rate of reaction from the results gained in the first 5 seconds as this will be the point where the greatest volume of gas evolves. I tried to keep this constant by making sure I swirled the conical flask evenly.

Average of the height of bubble formed: For this reason, I will take the following safety precautions:. The different concentrations of hydrogen peroxide that I made could not have been experoment accurate because this would have meant that the volume of gas evolved would have increased in equal steps, which it did not.

However, some oxygen was displaced in the gas syringe and I had to solve this by subtracting this small amount from the volumes produced in each of the reactions. Investigate the effect of substrate concentration on the rate of activity of the enzyme catalase. The balance proved to be the biggest apparatus error and this would have been much bigger if I had used only 0. Some of the teammates were not aware of the fact that they should measure the foam right after 2 min.


This site uses cookies: The plotted results on the graph produce a straight line of best fit to begin with which then goes into a curve of steadily decreasing gradient.

catalase and hydrogen peroxide experiment coursework

Catalase causes this catalytic effect of the enzyme Evaluation The rate of experinent of hydrogen peroxide is tested and observed with increasing concentration of catalase from potato skin.

Represent the results in a graph in order to work out the gradient and make a conclusion based on the evidence you have obtained.

Size Gcse Coursework Biology Potato Catalase Hydrogen Peroxide

Data presentation Perooxide 1. This is because at higher concentration there are more molecules of substrate, so more collisions take place, resulting in more enzyme-substrate complexes being formed.

catalase and hydrogen peroxide experiment coursework

courseowrk November 7, at However, I also believed that if I halved the concentration then the rate of reaction volume of oxygen produced would also be halved, and so the rate would be proportional to the concentration. When this saturation point is reached, then adding extra substrate will make no difference. Follow Us on the Move. Investigating the rate of reaction of the enzyme catalase Stopwatch was started the moment catalase was xoursework in the test tubes, and stopped after two minutes.

The Effect Of Substrate Concentration On The Activity Of The Enzyme Catalase

Although I did not check for gas leaks beforehand, there was good agreement between my replicates. What I know is that this type of reaction should be catabolism and not anabolism as you said.


In all test tubes, catalase was added lastly. I would expect this graph to be linear as this would show that as the concentration increases, the time taken for a set volume of gas would decrease. Results I carried out the above experiment and these results were obtained. Repeat all the tests at least three times so exxperiment an average can be obtained.

size gcse coursework biology potato catalase hydrogen peroxide

This is used to identify particular browsers or devices when the access the service, and is used for security reasons. If you need this or any other sample, we can send it to you via email. Finally, our group should not forget about human error. The volume of gas in the test tube to start with is slightly affected by the amount which the bung is pushed down each time, if the bung is pushed down further then the volume in the tube xoursework be less so the 30cm3 of gas is reached faster.

I consequently reduced the mass of yeast to 0. Enzyme Essay – digitaltermpapers. Subscribe to our Fortnightly Newsletter.

Size Gcse Coursework Biology Potato Catalase Hydrogen Peroxide

I was wondering how i could reference this page in APA format. Competitive inhibitors are the molecules which have a shape similar to the substrate, so they fit into the active site of the enzyme.

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